丙酮酸(Pyruvate)定量分析試劑盒檢測精準可靠!

丙酮酸鹽在糖酵解等過程中有重要作用: 它可以在細胞溶質葡萄糖進行糖酵解的過程中,由磷酸烯醇式丙酮酸產生。丙酮酸鹽可以繼續進入檸檬酸循環繼續氧化分解,轉化為碳水化合物或通過乙酰CoA 轉化為脂肪酸。高水平的丙酮酸鹽提示肝臟疾病和遺傳性代謝紊亂。丙酮酸鹽也被用來作為減肥刺激劑。在無氧呼吸中丙酮酸可以轉化為乳酸或者乙醇。

 

Pyruvate-pathway

 

作為專業性的產品供應公司,艾美捷科技為您推薦,可同時用于比色法和熒光法的丙酮酸定量分析試劑盒:K609-100

 

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產品名稱及描述 貨號 產品說明
Pyruvate Colorimetric/Fluorometric Assay Kit K609-100 詳情

* 細胞或組織樣品需做去蛋白處理(會消耗丙酮酸),詳情請見說明書

* 基于客戶儀器設備,自行選擇比色法或者熒光法即可

 

丙酮酸檢測分析試劑盒原理:

 

丙酮酸鹽檢測試劑盒提供了一種簡單、直接和可自動化操作的程序來測量不同生物學標本中丙酮酸的含量。適用標本類型包括:食物、細胞、培養基和發酵培養基。丙酮酸鹽檢測試劑盒基本原理如下:丙酮酸鹽被丙酮酸酶氧化,在酶反應過程中產生顏色(λ= 570 nm)或者熒光(Ex/Em = 535/587 nm),可使用酶標儀或熒光計檢測到。光的強度與丙酮酸含量是成正比的,因此通過檢測光的強度即可精確的得到丙酮酸的含量。試劑盒檢測范圍為1-10 uM丙酮酸。

 

pyruvate-standard-curve

 

《比色法和熒光法標準曲線展示》

 

丙酮酸檢測分析試劑盒發表文章:

 

Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.

Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J. Biol. Chem., Sep 2015; 290: 23240 - 23253.

Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci. Rep., Jun 2015; 35: e00214.

Chettimada et al., Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.Am J Physiol Lung Cell Mol Physiol, Feb 2015; 308: L287 - L300.

Cho, S.C. et. al. DDS, 4,4'-diaminodiphenylsulfone, extends organismic lifespan. PNAS, Nov 2010; 107: 19326 - 19331.

Luitpold Fried et al., Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli. J. Bacteriol., Feb 2013; 195: 807 - 815.

McNeely, K. et al. Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium. Appl. Envir. Microbiol., 2010; 10.1128/AEM.00862-10

Min You et al., Ethanol administration exacerbates the abnormalities in hepatic lipid oxidation in genetically obese mice. Am J Physiol Gastrointest Liver Physiol, Jan 2013; 304: G38 - G47

Müller et al., Engineering of Ralstonia eutropha H16 for Autotrophic and Heterotrophic Production of Methyl Ketones. Appl. Envir. Microbiol., Jul 2013; 79: 4433 - 4439.

Negi et al., A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation. Plant Physiology, Feb 2014; 164: 922 - 934.

Odet, F. et. al. Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm. Biol Reprod, Sep 2011; 85: 556 - 564.

Palamiuc et al., A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis. EMBO Mol Med., May 2015; 7: 526 - 546.

Park et al., Changes in Pyruvate Metabolism Detected by Magnetic Resonance Imaging Are Linked to DNA Damage and Serve as a Sensor of Temozolomide Response in Glioblastoma Cells.Cancer Res., Dec 2014; 74: 7115 - 7124.

 

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lamin b1tel

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